Counter-current immunoelectrophoresis for the diagnosis of pneumococcal chest infection.
نویسندگان
چکیده
Counter-current immunoelectrohporesis is a rapid sensitive method for detecting pneumococcal capsular antigens in sputum. A result can be obtained within 45 minutes. The optimum conditions for performing the test are given. Counter-current immunoelectrophoresis works with all the 33 pneumococcal antigens tested except type 14. Better precipitin lines are obtained when the gel-support is acid (pH 6-6) than when it is alkaline (pH 8-6). Omniserum is as effective as group-specific sera for the identification of pneumococcal capsular antigens. The findings suggest that pneumococcal infection was present in 44% of 300 unselected suspected chest infections. Culture for pneumococci was positive in only 15% of these cases. The clinical importance of these findings is still being studied but our experience has shown that patients with chest infections should have effective antipneumococcal antibiotics as part of their regimen and that the laboratory diagnosis may be made quickly and accurately by counter-current immunoelectrophoresis.
منابع مشابه
Countercurrent immunoelectrophoresis: improved detection of pneumococcal capsular antigens in sputum by incorporation of a carboxylated derivative of phenyl boronic acid.
Counter-current immunoelectrophoresis (CIEP) is now a well-recognised technique for the detection of pneumococcal antigens in body fluids. Much has been reported on its use in pneumococcal chest infections, but reports vary as to its ability to detect all pneumococcal types. Pneumococcal capsular types 7 and 14 could not be detected by Kenny et al. (1972) or Coonrod and Rytel (1973). El-Refaie ...
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عنوان ژورنال:
- Journal of clinical pathology
دوره 28 10 شماره
صفحات -
تاریخ انتشار 1975